《植物生理学报》 2010, 46(7): 659-663

番茄LeCIPK3 的克隆及非生物胁迫诱导的表达分析

李莉, 李毅, 王长春, 王锋青, 杨玲*

浙江师范大学化学与生命科学学院, 浙江金华321004

通信作者：杨玲；E-mail: yangl@zjnu.cn；Tel: 0579-82282396

摘　要：

CIPK 是植物钙感受器蛋白钙调磷酸酶 B 类似蛋白特定靶向的一类丝氨酸 / 苏氨酸蛋白激酶。采用同源基因克隆法, 在番茄叶中克隆了一个与AtCIPK3 处于同一亚组的基因, 命名为LeCIPK3。表达分析表明, LeCIPK3 主要在根和花中表达, 在叶中的表达受脱落酸、聚乙二醇及低温的诱导, 可能在番茄多种胁迫抗性中起重要作用。

关键词：番茄; CIPK; 克隆; 表达; 非生物胁迫

收稿：2010-01-22   修定：2010-05-07

资助：国家自然科学基金(30700519)、浙江省自然科学基金(Y3090645)和浙江省大学生科技创新项目。

Cloning and Expression Analysis of LeCIPK3 in Tomato Seedlings under Abiotic Stress

LI Li, LI Yi, WANG Chang-Chun, WANG Feng-Qing, YANG Ling*

College of Chemistry and Life Sciences, Zhejiang Normal University, Jinhua, Zhejiang 321004, China

Corresponding author: YANG Ling; E-mail: yangl@zjnu.cn; Tel: 0579-82282396

Abstract:

CIPK (calcineurin B-like proteins-interacting protein kinase), a plant-specific family of serine-threonine protein kinase, is specifically targeted by calcineurin B-like proteins. The degenerate primers were designed on the basis of a highly conserved region in N terminal of CIPK family. A CIPK gene, which was assigned into the same sub-group as AtCIPK3, was cloned from tomato leaves and named as LeCIPK3. The expression analysis showed LeCIPK3 had highest expression level in flowers and roots under the normal condition, while it could be induced in leaves by abscisic acid, polyethylene glycol and cold. These results suggested that LeCIPK3 might be involved in many types of abiotic stresses.

Key words: Lycopersicon esculentum; CIPK; clone; expression; abiotic stress